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  Section A: Allele-specific primers of SNPs in the Database

 

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(Continue Section C if you wish to adjust primer selection parameters then click submit below)


  Section B: User-defined Input


  Section C: Primer Selection Parameters

 

Primer Length Optimal: Min: Max:
GC% Optimal: Min: Max:
Tm Optimal: Min: Max:
Max Tm Diff:
Max Self Complementarity:
Max 3' Self Complementarity:
Max PolyX in Primer:
Product Size Range Min: Max:
Number of Primer Pairs:

Primer Filtering:
In-silico PCR Filtering maxSize
Mispriming Filtering from Primer3 Library

 
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