WASP Web-based Allele Specific Primer design tool




Section A: Input

Mismatch at the penultimate position of primers:

Section B: Parameters for primer design

Primer Length optimal:
Min:
Max:
GC% optimal:
Min:
Max:
Tm optimal:
Min:
Max:
Max Tm Diff:
Max Self Complementarity:
Max 3' Self Complementarity:
Max polyX in primer:
Number primer pairs to return:
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